Different aspects in manipulating overlapped spectra used for the analysis of trimebutine maleate and structure elucidation of its degradation products

Future Journal of Pharmaceutical Sciences

Table 1 Regression parameters and validation sheet for the determination of pure samples of trimebutine maleate by the proposed UV-spectrophotometric methods

Parameters		DW	D²	RD	CC-SS
Range^a (μg/mL)		5.00–60.00
Linearity	Slope	0.0187	0.0119	0.0589	0.0287
	Intercept	+ 0.0023	+ 0.0029	+ 0.0029	− 0.0038
	Correlation coefficient (r)^b	1.0000	1.0000	1.0000	1.0000
Mean ± SD		99.97 ± 0.40	100.36 ± 0.58	99.90 ± 0.42	100.15 ± 0.45
Accuracy (mean ± SD)		99.90 ± 0.61	100.96 ± 0.46	99.74 ± 0.89	100.74 ± 0.53
Precision	Repeatability^c	0.361	0.348	0.692	0.649
Precision	Intermediate precision^d	0.828	0.699	0.858	0.813
Specificity (mean ± SD)		99.59 ± 0.19	100.37 ± 0.54	99.89 ± 0.59	99.54 ± 052

^aSeven calibration points in μg/mL, average of three experiments
^br = √R²
^cRelative standard deviation (RSD)% average of three different concentrations repeated three times within the same day (10.00, 30.00, 50.00 μg/mL) = (intra-day precision)
^dRelative standard deviation (RSD)% average of three different concentrations repeated three times each on three successive days (10.00, 30.00, 50.00 μg/mL) = (inter-day precision)