Table 1 Acceptance criteria for UPLC-MS/MS
Characteristics | UHPLC |
|---|---|
Particle size | < 2 μm |
Maximum back pressure | 1000 bars |
Analytical column | UHPLC BEH C18 |
Column dimensions | 50 × 2.1 mm |
Injection volume | 2 μL |
Column temperature | 65 °C |
Total run time | 1.5 min |
USP resolution | 3.4 |
Plate count | 7500 |
Flow rate | 0.6 mL/min |