Skip to main content

Table 1 Acceptance criteria for UPLC-MS/MS

From: Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

Characteristics UHPLC
Particle size < 2 μm
Maximum back pressure 1000 bars
Analytical column UHPLC BEH C18
Column dimensions 50 × 2.1 mm
Injection volume 2 μL
Column temperature 65 °C
Total run time 1.5 min
USP resolution 3.4
Plate count 7500
Flow rate 0.6 mL/min