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Table 6 Separation of isomers studies using UHPLC

From: Ultra-high performance liquid chromatography-MS/MS (UHPLC-MS/MS) in practice: analysis of drugs and pharmaceutical formulations

Drugs/compounds Column Mobile phase system Mode of analysis Detector Extraction method Ref
Flavanone, Hesperetin, and Naringenin Chiralpak AD-3R Methanol Isocratic DAD SPE [165]
Flavanone, Naringenin, and Hesperetin Chiralpak AD-3R Methanol Isocratic Triple-quadrupoleMass spectrometer SPE [166]
Phenylethylamines and cathinones BEH phenylCSH Fluro-phenyl 80 mM phosphate buffer (A) and acetonitrile or methanol (B) Isocratic/gradient PDA [167]
Besifoxacin Chiralpak IE-3 Dichloromethane: isopropyl alcohol: trifuoroacetic
acid: diethylamine (90:10:0.3:0.05 v/v/v/v)
Isocratic UV-Visible detector - [168]
Warfarin ZORBAX RRHD Eclipse C18, SB-CN, SB-C8 and SB-Phenyl Hypersil Gold AQ and Gold PFP 0.1% Phosphoric acid in water (A) 10 mM ammonium acetate (pH 6.5) (B) and 5mM ammonium phosphate dibasic in water (pH 8.0) (C). 95:5 to 5:95 % A, B or C. Gradient PDA --- [169]
Debenzazepine and Carbamazepine analogs, Hypersil Gold PFP 2 mM Ammonium formate (A) and acetonitrile (B) 0-0.2 min, 80% (A): 20% (B); 0.2–0.8 min, 5% (A) :95% (B); and back to 80% (A): 20% (B) in 0.5 min Gradient PDA [170]
Xanthine metabolites SB-Phenyl 0.1 H3PO4 in water: acetonitrile Gradient PDA
Steroid analogs Eclipse Plus C18 2 mM Ammonium formate in water (pH 3.5) and acetonitrile (65:35 to 5:95 in 2.0 min) Gradient PDA
Nicotine analogs SB-CN 5 mM Ammonium phosphate diabasic in water: acetonitrile Gradient PDA
Glucuronide analogs SB-CN 150 mM sodium percholrate in 0.02 % perchloric acid: acetonitrile Gradient PDA
Rasagiline Chiralpak AGP Ammonium acetate: isopropyl alcohol (90:10, v/v) Isocratic PDA -- [171]
Trantinterol BEH C18 3 mM ammonium acetate (A) and acetonitrile (B). 0–4 min, 80-20 %(A); maintained at 70% (A) for 2.5 min Gradient Triple-quadrupolemass spectrometer LLE [172]
SCH 503034 BEH C18 A1-Methanol: water (5:95) with 40 mM PFPA and A2- methanol: water (95:5) with 40 mM PFPA.
B1- Methanol: water (5/95) with 4 mM of ammonium acetate and B2-methanol: water (95/5) with 4 mM of ammonium acetate
Gradient Triple-quadrupolemass spectrometer PP [173]
Ranolazine BEH RP18 (A) Acetonitrile: sodium dihydrogen phosphate (pH 7.3; 0.01 M)-Triethylamine (10:90:0.1, v/v/v).
(B)- Acetonitrile-mobile phase-A (55:45, v/v).0.01 min, 17 % (B); 1.5 min, 17%(B); 3.5 min, 45%(B); 5.5 min, 60% (B); 8 min, 65 %(B); 12 min, 70% (B); 13 min, 95% (B); 15 min, 95 %(B); 15.5 min, 17% (B); and 18 min, 17%(B)
Gradient PDA [174]
Rivastigmine BEH Phenyl (A) Acetonitrile: disodium hydrogen orthophosphate (pH 7.5; 0.01 M)-Triethylamine (10:90:0.1 v/v/v).
(B) Acetonitrile: water (80:20 v/v) 0.01 min, 5.0 %(B); 5.0 min, 35.0 % (B); 8.0 min, 60.0% (B);8.1 min, 5.0 % (B);10.0 min, 5.0%(B).
Gradient PDA [175]
Benzoic acid Thermo Syncronis C18 0.1% formic acid in water (A) and methanol (B) 0–1.5 min, 15–46 %(B); 1.5–1.9 min, 46% (B); 1.9–4.5 min, 15% (B) Gradient Triple-quadrupolemass spectrometer LLE [176]
Phenylmercapturic acid and Benzyl mercapturic acid BEH C18 0.1% acetic acid in water (A) and methanol (B). 0–1.0 min, 80% (A); 1.01–6.00 min, 80–70%(A); 6.00 min, 70–80 % (A) Gradient Triple-quadrupolemass spectrometer LLE [177]
Abacavir BEH C18 Water: Acetonitrile (90:10 v/v) Isocratic UV-Vis and PDA [178]
Stimulants agents BEH C18 Water (A) and acetonitrile with 0.01% formic acid (B) (50:50 v/v) Four types of gradient program were used:
1. Gradient A/B: 0 min 1% B, increase to 3% B in 4.2 min, increase to 90%B in 3.8 min, 90% B for 0.5 min, decrease to 1% B in 0.1 min, and equilibration for 1.4 min.
2. Gradient C: 1% B for 1 min, increase to 15% B in 7 min, increase to 90% B in 1 min, 90% B for 1 min, decrease to 1% B in 0.1min, and equilibration for 1.9 min.
3. Gradient D: 5% B for 1.2 min, increase to 90% B in 16.8 min, 90% B for 0.7 min, decrease to 5% B in 0.1 min, and equilibration for 1.2 min.
4. Gradient E/F: 1% B for 1.2 min, increase to 90% B in 6.8 min, 90%B for 0.7 min, decrease to 1% B in 0.1 min, and equilibration for 1.2 min
Gradient Triple-quadrupolemass spectrometer [179]
Vigabatrin Eclipse Plus C18 column A-10 mM ammonium formate (pH 3.0 adjusted with formic acid and methanol (B), 0–1 min, 10–10% (B); 1–8 min, 10–16% (B), 8–9 min, 16–100% (B), 9–11 min, 100% (B) Gradient Triple-quadrupolemass spectrometer LLE [180]
Zoxamide Lux Amylose-2 Acetonitrile: water (70:30 v/v) Isocratic Triple-quadrupolemass spectrometer QuEChERS [181]