Table 2 Chromatographic conditions used for the method
Sr. No. | Parameter | Chromatographic conditions |
|---|---|---|
1. | Stationary phase | Aluminium plates pre-coated with silica gel 60 F254 (10 × 10 cm) |
2. | Mobile phase | Toluene:ethyl acetate:0.1 glacial acetic acid (8:2:0.1 v/v/v) |
3. | Chamber saturation time | 30 min |
4. | Temperature | Room temperature (25 + 2 °C) |
5. | Migration distance | 90 mm |
Application parameters | ||
6. | Syringe | 100 μl |
7. | Application rate | 100 nl/sec |
8. | Band width | 6 mm |
9. | Distance from the plate edge | 15 mm |
10. | Distance from the bottom of the plate | 15 mm |
11. | Distance between the bands | 10 mm |
Scanning parameters | ||
12. | Wavelength of detection | 226.5 nm |
13. | Slit dimension | 4.00 × 0.30 mm |
14. | Scanning speed | 20 mm/sec |
15. | Lamp | D2 |
16. | Measurement mode | Absorption |