Fig. 3

Induction of apoptosis by Hst, Dox, and their combination in 4 T1 cells. A total of 1.5 × 10⁵ cells were grown on 6-well plates and incubated for 24 h. A concentration series of Hst, Dox, and their combination was administered for the following 24 h. Cells were then collected and stained with an Annexin-V-FLUOS Staining Kit before measurement with a flow cytometer. a Histogram of cell death profile, i.e., living cells, early apoptotic cells, late apoptosis, and necrosis. b Bar chart of cell death distribution with early and late apoptosis, as well as necrosis. Asterisk and number sign indicate significance to Dox and Hst, respectively (p < 0.05)