Fig. 5From: Putative anti-inflammatory, antioxidant, and anti-apoptotic roles of the natural tissue guardian methyl palmitate against isoproterenol-induced myocardial injury in ratsImmunohistochemical analysis of Bax and Bcl-2 in heart sections (magnification × 400). a and e Control group showing minimal Bax expression and maximal Bcl-2 expression respectively. b and f MP group showing minimal Bax expression and maximal Bcl-2 expression respectively. c and g ISO-intoxicated group showing extensive heavy Bax expression and minimal Bcl-2 expression respectively. d and h MP-pretreated group showing mild Bax expression and extensive Bcl-2 expression respectively. i and j Quantitative image analysis for immunohistochemical staining of Bax and Bcl-2 respectively expressed as optical densities (OD) across 10 different fields for each rat section. k The ratio of Bax to Bcl-2 expression in the heart of different groups. Data are presented as mean ± SD (n = 10); a or b: significantly different from control or ISO groups respectively at P < 0.05 using one-way ANOVA followed by Tukey as post hoc test. MP methyl palmitate, ISO isoproterenol, Bax Bcl-2-associated X, Bcl-2 B cell lymphoma 2Back to article page