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Table 2 Thin layer chromatography of hydro-methanolic extract of Sterculia urens Roxb. root

From: In vitro antioxidant and antimicrobial potential of Sterculia urens Roxb. root extract and its bioactive phytoconstituents evaluation

Compound name Solvent system Confirmatory test Spot band colour Rf value
Visible light Ultraviolet 365 nm
Phenolics Toluene to acetone (9:1) Alcoholic ferric Chloride Brownish-yellow NII 0.92
Alkaloid Diethylamine to methanol (8:1:1) Dragendorff reagent Yellow Blue, red 0.72
Flavonoid Glacial acetic acid to ethyl acetate to formic acid to water (0.5:10:0.5:1.3) 10% methanolic sulfuric acid Brown Orange, red, green 0.84
Steroid Butanol to methanol to water (16:4:0.2) 10% methanolic sulfuric acid Purple Blue, red 0.74
Tannins Methanol to chloroform: to water (35:65: 10) Vanillin solution Brownish green Blue, red 0.52
  1. (NII negligible)