Table 2 Thin layer chromatography of hydro-methanolic extract of Sterculia urens Roxb. root
Compound name | Solvent system | Confirmatory test | Spot band colour | Rf value | |
|---|---|---|---|---|---|
Visible light | Ultraviolet 365 nm | ||||
Phenolics | Toluene to acetone (9:1) | Alcoholic ferric Chloride | Brownish-yellow | NII | 0.92 |
Alkaloid | Diethylamine to methanol (8:1:1) | Dragendorff reagent | Yellow | Blue, red | 0.72 |
Flavonoid | Glacial acetic acid to ethyl acetate to formic acid to water (0.5:10:0.5:1.3) | 10% methanolic sulfuric acid | Brown | Orange, red, green | 0.84 |
Steroid | Butanol to methanol to water (16:4:0.2) | 10% methanolic sulfuric acid | Purple | Blue, red | 0.74 |
Tannins | Methanol to chloroform: to water (35:65: 10) | Vanillin solution | Brownish green | Blue, red | 0.52 |