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Table 3 Stress study of hydrocortisone and clotrimazole assay for experimental topical cream at 40 °C temperature and sunlight

From: Analytical method development and validation of hydrocortisone and clotrimazole in topical dosage form using RP-HPLC

Working standards

Initial analysis (%)

40 °C ± 2/75% ± 5 RH (3 months)

40 °C ± 2/75% ± 5 RH (6 months)

Sunlight 10 h (28–32 °C)

Hydrocortisone

99.89

99.47

98.63

98.56

Clotrimazole

99.87

98.52

98.45

98.67