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Table 1 Column, mobile phase systems, and internal standards used for the optimization of the HPLC method

From: Novel approaches for determination of antiretroviral reverse transcriptase inhibitor agent in commercial dosage forms by using spectrofluorimetric, first derivative spectrophotometric, and HPLC methods

Column

Mobile phase*

Internal standard

Thermo Hypersil Phenyl 250 × 4.6 mm 5 μ

ACN:MeOH

Caffeine

ACN:MeOH:Diethylamine

Hydrochlorothiazide

ACN:0.5% Formic acid

Zanamivir

Waters Xterra RP8 5 μ 250 × 4.6 mm

ACN:MeOH

Oseltamivir

ACN:0.5% Formic acid

Lopinavir

Wakosil-II 5 C18 AR, 4.6 × 250 mm

ACN:0.5% Formic acid

Ritonavir

  1. *Mobile phase components were at different rates