From: Novel approaches for determination of antiretroviral reverse transcriptase inhibitor agent in commercial dosage forms by using spectrofluorimetric, first derivative spectrophotometric, and HPLC methods
Parameters
HPLC
Reference value [36, 37]
Retention time, tr
2.64
─
Capacity factor, K′
3.19
K′ > 2.0
Resolution, Rs
3.806
Rs > 2.0
Selectivity, α
4.437
α > 1.0
Theoretical plates, N (plates/column)
11207
N > 2000
Tailing factor, T
1.191
T ≤ 2.0
RSD(%)
0.12
RSD ≤ 1%