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Table 2 System performance parameters obtained by HPLC for tenofovir

From: Novel approaches for determination of antiretroviral reverse transcriptase inhibitor agent in commercial dosage forms by using spectrofluorimetric, first derivative spectrophotometric, and HPLC methods

Parameters

HPLC

Reference value [36, 37]

Retention time, tr

2.64

Capacity factor, K

3.19

K′ > 2.0

Resolution, Rs

3.806

Rs > 2.0

Selectivity, α

4.437

α > 1.0

Theoretical plates, N (plates/column)

11207

N > 2000

Tailing factor, T

1.191

T ≤ 2.0

RSD(%)

0.12

RSD ≤ 1%

  1. RSD(%) relative standard deviation of six replicate injections’ peak areas