Table 3 Analytical parameters for the analysis of Tenofovir by the purposed spectrofluorimetric, first derivative spectrophotometric, and HPLC methods

Concentration range (μg/mL)

4.72–15.75

10.0–28.0

10.00–100.00

Slope (a)

39.002

0.9827

0.1398

Intercept (b)

158.85

0.0073

− 0.0387

Correlation coefficient (r)

0.9997

0.9998

0.9995

Determination coefficient (r²)

0.9994

0.9996

0.9990

Standard deviation of the slope (S_a)

1.64

1.00

0.0016

Standard deviation of the intercept (S_b)

17.85

2.79

0.0982

Limit of detection (LOD) (μg/mL)

1.51

1.31

2.32

Limit of quantitation (LOQ) (μg/mL)

4.58

4.53

7.02