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Table 2 Validation parameters and statistical data with regression analysis of satranidazole (STZ) (n = 10)

From: Improved comprehensive analytical method for assessment of satranidazole in drug and product

Solvent

Method

Regression analysis

Validation parameters

Slope

(S.Ea)

95% CLb of slope

Intercept (S.Ec)

95% CL of icptd

R2

95% CL of te

LODf

(μg ml−1)

LOQg

(μg ml−1)

Methanol

A

0.048

(0)

–

0.027

(0.0010)

3.94E−05

0.998

0.3804

0.0859

0.2604

B

0.027

(0.0005)

1.56E−05

0.044

(0.0049)

0.000126

0.999

0.1449

0.4925

1.4814

C

 

7.83E−05

0.308

(0.1493)

0.004484

0.999

0.0630

1.3961

4.2307

Ethanol

A

 

0.077072

0.118

(0.0078)

0.000387

0.999

0.5971

1.9328

5.9725

B

0.015

(0)

0.337

(0.0030)

0.107

(0.0007)

6.00E−05

0.999

0.8314

0.4210

1.2758

C

0.171

(0.0064)

0.021

(0.0031)

1.259

(0.1947)

0.00616

0.999

0.5142

3.7901

11.4084

DMF

A

0.056

(0.0104)

0.00032

0.095

(0.0227)

0.00070

0.999

0.4931

1.0690

3.2178

B

0.044

(0)

–

0.092

(0.0024)

5.91E−05

0.999

0.0873

0.18

0.5418

C

0.647

(0.0010)

0.03354

0.855

(0.0700)

0.002667

0.999

0.1877

0.3570

1.0746

Benzene

A

0.037

(4.6E-05)

0.00101

0.040

(0.0013)

0.029218

0.999

0.1116

0.1203

0.3623

B

0.026

(2.5E−05)

0.00096

0.044

(0.0006)

0.025024

0.999

0.3036

0.0805

0.2495

C

0.192

(3.6E−05)

0.00122

0.347

(0.0019)

0.06532

0.999

0.6097

0.0332

0.1002

Acetonitrile

A

0.105

(0.0047)

0.000128

0.013

(0.0225)

0.00058

0.999

0.1675

0.7071

2.1285

B

0.064

(0.0018)

6.46E−05

0.007

(0.0117)

0.00036

0.999

0.7152

0.6037

1.8174

C

0.871

(0.0441)

0.001319

0.087

(0.2136)

0.006256

0.999

0.1083

0.8092

2.4359

Meth chloride

A

0.043

(0)

–

− 0.006

(0.0060)

0.00018

0.999

0.0171

0.4662

1.4033

B

0.026

(0.0004)

1.70E-05

− 0.039

(0.0061)

0.000231

0.999

0.1455

0.7742

2.3304

C

0.410

(0.0303)

0.000537

− 0.513

(0.1474)

0.005395

0.999

0.7257

1.1863

3.5710

DDW

A

0.033

(0.0011)

4.92E-05

(7E−06)

(2.2E−06)

0.00076

0.999

0.0764

0.0002

0.0006

B

0.021

(0)

–

0.004

(5.5E−05)

0.00012

0.999

0.0245

0.0086

0.0267

C

0.281

(0.0091)

0.00091

− 0.031

(0.00124)

1.92E−05

0.999

0.0875

0.0145

0.0451

  1. aStandard error of mean
  2. bRegression coefficient slopee
  3. cStandard error
  4. dRegression coefficient
  5. eTheoretical values (95% confidence limit), t = 2.225
  6. fDetection limit
  7. gQuantification limit