Improved comprehensive analytical method for assessment of satranidazole in drug and product

Mazumder, Rana; Mahanti, Beduin; Majumdar, Subhabrota; Pal, Rabindranath; Chowdhury, Ashok Dhar

doi:10.1186/s43094-020-00072-8

Future Journal of Pharmaceutical Sciences

Table 3 Accuracy and precision analysis data

From: Improved comprehensive analytical method for assessment of satranidazole in drug and product

Solvent	Method	Level	Conc -μg/ml	Estimated concentrations (μg ml⁻¹) by linear regression equation			Mean % recovery (± S.D.)	Accuracy ^d (% RE)
Solvent	Method	Level	Conc -μg/ml	Range	A.M. ± S.D.	%RSD	Mean % recovery (± S.D.)	Accuracy ^d (% RE)
Benzene	A	LC^a	8.75	8.91–8.59	8.74 ± 0.0862	0.987	99.912 ± 0.986	− 0.088
		IC^b	17.5	17.73–17.24	17.51 ± 0.1186	0.678	100.098 ± 0.677	0.098
		HC^c	26.25	26.71–25.99	26.49 ± 0.2013	0.767	100.95 ± 0.767	0.95
	B	LC^a	11.25	11.36–11.17	11.27 ± 0.0635	0.564	100.198 ± 0.565	0.198
		IC^b	22.5	22.89–22.32	22.71 ± 0.1283	0.565	100.934 ± 0.570	0.934
		HC^c	33.75	34.42–33.07	33.75 ± 0.2690	0.797	100.005 ± 0.797	0.005
	C	LC^a	8.75	8.92–8.57	8.75 ± 0.0554	0.634	100.023 ± 0.634	0.023
		IC^b	17.5	17.81–17.19	17.55 ± 0.1179	0.672	100.309 ± 0.674	0.309
		HC^c	26.25	26.50–25.99	26.51 ± 0.086	0.327	100.991 ± 0.330	0.991
Methenchloride	A	LC^a	7.5	7.61–7.42	7.49 ± 0.0575	0.767	99.991 ± 0.766	− 0.009
		IC^b	15	15.28–14.79	14.99 ± 0.1316	0.878	99.978 ± 0.877	− 0.022
		HC^c	22.5	22.72–22.29	22.49 ± 0.0886	0.394	99.959 ± 0.393	− 0.041
	B	LC^a	11.25	11.43–11.24	11.34 ± 0.0665	0.587	100.847 ± 0.591	0. 847
		IC^b	22.5	22.69–22.09	22.36 ± 0.1149	0.514	99.416 ± 0.510	− 0. 584
		HC^c	33.75	34.35–33.39	33.95 ± 0.2027	0.597	100.619 ± 0.600	0. 619
	C	LC^a	7.5	7.58–7.49	7.55 ± 0.0224	0.298	100.708 ± 0.300	0. 708
		IC^b	15	15.05–14.68	14.85 ± 0.0692	0.466	99.053 ± 0.461	− 0. 947
		HC^c	22.5	22.93–22.13	22.72 ± 0.1969	0.867	100.986 ± 0.875	0. 986
Acetonitrile	A	LC^a	5.75	5.79–5.75	5.77 ± 0.0205	0.356	100.516 ± 0.357	0. 516
		IC^b	7.5	7.61–7.39	7.42 ± 0.0501	0.675	99.03 ± 0.668	− 0. 969
		HC^c	9.25	9.34–9.14	9.23 ± 0.0622	0.675	99.83 ± 0.673	− 0. 170
	B	LC^a	5	5.09–4.98	5.04 ± 0.0213	0.423	100.890 ± 0.426	0. 890
		IC^b	10	10.17–9.85	10.01 ± 0.066	0.667	100.180 ± 0.668	0. 180
		HC^c	15	15.24–14.77	15.06 ± 0.1268	0.842	100.458 ± 0.845	0. 458
	C	LC^a	5.75	5.83–5.66	5.76 ± 0.0485	0.841	100.345 ± 0.843	0.345
		IC^b	7.5	7.50–7.05	7.44 ± 0.0256	0.587	99.31 ± 0.582	− 0. 690
		HC^c	9.25	9.42–9.25	9.32 ± 0.0427	0.459	100.801 ± 0.462	0. 801
DMF	A	LC^a	5	5.04–4.99	5.02 ± 0.009	0. 187	100.409 ± 0.187	0. 409
		IC^b	10	10.12–9.92	10.07 ± 0.0247	0. 246	100.763 ± 0.247	0.763
		HC^c	15	15.19–14.79	14.96 ± 0.0772	0. 516	99.74 ± 0.514	− 0. 256
	B	LC^a	7.25	7.41–7.21	7.31 ± 0.0721	0. 987	100.854 ± 0.995	0.854
		IC^b	12.5	12.54–12.42	12.48 ± 0.0293	0. 236	99.884 ± 0.235	− 0. 116
		HC^c	17.75	17.98–17.75	17.80 ± 0.1267	0.712	100.325 ± 0.714	0.325
	C	LC^a	5	5.04–4.95	5.01 ± 0.0188	0. 375	100.387 ± 0.376	0. 387
		IC^b	10	10.15–9.99	10.08 ± 0.0269	0. 267	100.834 ± 0.269	0. 834
		HC^c	15	15.20–14.86	15.03 ± 0.0598	0. 398	100.256 ± 0.399	0. 256
Ethanol	A	LC^a	11.25	11.32–11.24	11.29 ± 0.0142	0. 126	100.423 ± 0.126	0. 423
		IC^b	22.5	22.94–22.19	22.69 ± 0.1853	0. 817	100.856 ± 0.823	0. 856
		HC^c	33.75	33.71–33.48	33.44 ± 0.0913	0. 273	99.10 ± 0.270	− 0.897
	B	LC^a	16.25	16.41–16.15	16.30 ± 0.0950	0. 583	100.344 ± 0.585	0.344
		IC^b	32.5	32.65–32.63	32.64 ± 0.0061	0. 019	100.435 ± 0.019	0.435
		HC^c	48.75	49.48–48.55	49.06 ± 0.414	0.846	100.643 ± 0.851	0.643
	C	LC^a	11.25	11.72–11.32	11.34 ± 0.0857	0. 756	100.823 ± 0.762	0.823
		IC^b	22.5	22.91–22.38	22.67 ± 0.1079	0. 476	100.765 ± 0.479	0.765
		HC^c	33.75	33.93–33.58	33.83 ± 0.0669	0. 198	100.245 ± 0.198	0.245
Methanol	A	LC^a	7.5	7.63–7.34	7.56 ± 0.0308	0. 408	100.845 ± 0.411	0.845
		IC^b	15	15.11–14.79	14.95 ± 0.0569	0. 381	99.67 ± 0.379	− 0.325
		HC^c	22.5	22.93–22.10	22.63 ± 0.1527	0. 675	100.586 ± 0.678	0.586
	B	LC^a	10	10.19–9.89	10.08 ± 0.0771	0. 765	100.847 ± 0.771	0.847
		IC^b	20	20.40–19.64	20.01 ± 0.1565	0. 782	100.097 ± 0.782	0.097
		HC^c	30	30.53–29.59	30.15 ± 0.1957	0. 649	100.516 ± 0.652	0.516
	C	LC^a	7.5	7.65–7.38	7.55 ± 0.0673	0. 891	100.784 ±v0.897	0.784
		IC^b	15	15.22–14.76	15.06 ± 0.1211	0. 804	100.456 ± 0.807	0.456
		HC^c	22.5	22.94–22.07	22.47 ± 0.2151	0. 957	99.903 ± 0.956	− 0.097
DDW	A	LC^a	8.75	8.91–8.64	8.80 ± 0.0604	0. 686	100.658 ± 0.690	0.658
		IC^b	17.5	17.41–17.39	17.42 ± 0.0128	0. 074	99.548 ± 0.073	− 0.452
		HC^c	22.5	22.64–22.39	22.66 ± 0.0423	0. 187	100.752 ± 0.188	0.752
	B	LC^a	10	10.14–9.91	10.00 ± 0.097	0. 965	100.047 ± 0.970	0.047
		IC^b	20	20.25–20.01	20.10 ± 0.083	0. 416	100.547 ± 0.418	0.547
		HC^c	30	30.51–29.69	30.07 ± 0.2394	0. 797	100.243 ± 0.798	0.243
	C	LC^a	8.75	8.71–8.71	8.71 ± 0.0036	0. 043	99.65 ± 0.042	− 0.345
		IC^b	17.5	17.83–17.36	17.63 ± 0.1510	0. 857	100.753 ± 0.863	0.753
		HC^c	26.25	26.43–26.25	26.36 ± 0.0594	0. 475	100.435 ± 0.477	0.435

^aLower concentration (LC) = Q₁ = value corresponding to rank ¼(n + 1) in μg ml⁻¹
^bIntermediate concentration (IC) = Q₂ = value corresponding to rank ½(n + 1) in μg ml⁻¹
^cHigh concentration, corresponding to rank ¾(n + 1) in μg ml⁻¹
^d\( \% RE=100\times \frac{\left|\left\langle x\right\rangle -\mu \right|}{\mu}\cdot \left[\left\langle x\right\rangle =\mathrm{avg}\ \mathrm{of}\ \mathrm{obs}\ \mathrm{HC}=\kern0.5em {\mathrm{Q}}_3=\mathrm{value}\ \mathrm{values},\mu \kern0.5em =\kern0.5em \mathrm{central}\ \mathrm{tendency}\ \mathrm{of}\ \mathrm{obs}\ \mathrm{values}\right] \)
^eArithmetic mean = AM

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