Parameter | Modified condition | % RSD of peak area (NMT 2.0 %) | Theoretical plates* N (> 2000) | Tailing factor* (< 2.0) | Rs* (> 2.0) | |||
---|---|---|---|---|---|---|---|---|
ERTU | MET | ERTU | MET | ERTU | MET | |||
Flow rate (ml/min) | 0.9 | 0.893 | 1.209 | 12,959 | 10,911 | 1.17 | 1.17 | 7.96 |
1.0 | 0.531 | 0.382 | 11,679 | 9947 | 1.23 | 1.33 | 7.21 | |
1.1 | 0.949 | 1.113 | 11,564 | 9463 | 1.21 | 1.16 | 7.63 | |
ACN ratio in mobile phase Buffer: ACN (% v/v) | 70:30 | 1.377 | 1.039 | 11,949 | 11,751 | 1.26 | 1.15 | 9.2 |
65:35 | 0.531 | 0.382 | 11,679 | 9947 | 1.23 | 1.33 | 7.21 | |
60:40 | 1.360 | 1.293 | 12,741 | 9485 | 1.22 | 1.14 | 6.78 | |
Temperature (°C) | 25 | 0.912 | 1.278 | 13,396 | 9470 | 1.14 | 1.20 | 7.03 |
30 | 0.531 | 0.382 | 11,679 | 9947 | 1.23 | 1.33 | 7.21 | |
35 | 0.574 | 0.993 | 13,396 | 9516 | 1.21 | 1.17 | 7.01 |