Parameters | Normal condition | Change in condition | Mean area ± SD (n=3) | %RSD | ||||
---|---|---|---|---|---|---|---|---|
PER (4μg/mL) | IND (1.25μg/mL) | AML (5μg/mL) | PER (4μg/ml) | IND (1.25μg/ml) | AML (5μg/ml) | |||
Change in mobile phase ratio | Acetonitrile :Methanol :Water (30:20:50, v/v/v) pH 3.0 adjust with 1% OPA | 35:20:45, v/v/v | 96342.33 ± 1553.51 | 108733 ± 1697.85 | 184767 ± 3241.86 | 1.61 | 1.56 | 1.75 |
30:25:45, v/v/v | 95567.00 ±1185.70 | 108541 ± 1894.39 | 186363 ± 2687.21 | 1.24 | 1.75 | 1.44 | ||
Change in wavelength | 215 nm | 214 nm | 95599.00 ± 1436.38 | 108503 ± 1388.35 | 185474 ± 3323.43 | 1.50 | 1.28 | 1.79 |
216 nm | 95124.33 ± 1666.298 | 108227 ± 1511.45 | 186912 ± 30511.9 | 1.75 | 1.40 | 1.63 | ||
Change in temperature | 25°C | 30°C | 96040.67 ± 1481.23 | 108799 ± 1573.84 | 185215 ± 3063.14 | 1.54 | 1.45 | 1.65 |
20°C | 96643.00 ± 1463.39 | 107725 ± 1778.23 | 186250 ± 3507.47 | 1.51 | 1.65 | 1.88 | ||
Change in flow rate | 1.0 ml/min | 0.9 ml/min | 96182.67 ± 1385.97 | 108906 ± 1343.03 | 187229 ± 2509.65 | 1.44 | 1.23 | 1.34 |
1.1 ml/min | 96400.67 ± 1736.06 | 10258 ± 1499.96 | 187316 ± 2661.07 | 1.80 | 1.39 | 1.42 |