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Table 3. Robustness study of proposed method

From: A new RP–HPLC method for simultaneous quantification of perindopril erbumine, indapamide, and amlodipine besylate in bulk and pharmaceutical dosage form

Parameters

Normal condition

Change in condition

Mean area ± SD (n=3)

%RSD

PER (4μg/mL)

IND (1.25μg/mL)

AML (5μg/mL)

PER (4μg/ml)

IND (1.25μg/ml)

AML (5μg/ml)

Change in mobile phase ratio

Acetonitrile :Methanol :Water (30:20:50, v/v/v) pH 3.0 adjust with 1% OPA

35:20:45, v/v/v

96342.33 ± 1553.51

108733 ± 1697.85

184767 ± 3241.86

1.61

1.56

1.75

30:25:45, v/v/v

95567.00 ±1185.70

108541 ± 1894.39

186363 ± 2687.21

1.24

1.75

1.44

Change in wavelength

215 nm

214 nm

95599.00 ± 1436.38

108503 ± 1388.35

185474 ± 3323.43

1.50

1.28

1.79

216 nm

95124.33 ± 1666.298

108227 ± 1511.45

186912 ± 30511.9

1.75

1.40

1.63

Change in temperature

25°C

30°C

96040.67 ± 1481.23

108799 ± 1573.84

185215 ± 3063.14

1.54

1.45

1.65

20°C

96643.00 ± 1463.39

107725 ± 1778.23

186250 ± 3507.47

1.51

1.65

1.88

Change in flow rate

1.0 ml/min

0.9 ml/min

96182.67 ± 1385.97

108906 ± 1343.03

187229 ± 2509.65

1.44

1.23

1.34

1.1 ml/min

96400.67 ± 1736.06

10258 ± 1499.96

187316 ± 2661.07

1.80

1.39

1.42