Table 1 Optimized chromatographic condition
HPLC unit | Jasco AS-2055 plus series intelligent sampler |
|---|---|
Stationary phase (column) | Agilent Eclipsed XDB-C18, (4.6 × 250 mm) packed with 5 μm particles |
Detector | Photodiode detector (PDA) |
Mobile phase | ACN, phosphate buffer 2.5 pH (85:15) |
Detection wavelength (nm) | 252.0 |
Run time (min) | 6 |
Flow rate (ml/min) | 1.2 |
Volume of injection loop (μL) | 50 |
Column temperature | Ambient |