Method | RP-HPLC | Derivative spectrophotometry | |
---|---|---|---|
Wavelength, nm | 225.0 | 237.7 | 257.2 |
Working range (μg mL−1) | 10.0 -70.0 | 6.0 -30.0 | 6.0-30.0 |
Slope (μg mL−1) | 0.1116 | 0.0018 | 0.0011 |
Intercept | 0.0770 | 0.0015 | 0.0005 |
Correlation coefficient, R2 | 0.9998 | 0.9999 | 0.9997 |
LOD (μg mL−1) | 0.243 | 0.9623 | 0.5746 |
LOQ (μg mL−1) | 0.811 | 3.2075 | 1.5486 |