Table 4 Inter-day and Intra-day variability of RP-HPLC for determining VRT concentrations
Intra-day variability | Inter-day variability | |||
|---|---|---|---|---|
Added (μg/mL) | Recovery (%) | RSD % | Recovery (%) | RSD % |
RP-HPLC | ||||
30.0 | 100.51 | 0.19 | 98.96 | 0.39 |
40.0 | 99.97 | 0.53 | 99.49 | 0.57 |
50.0 | 101.5 | 0.18 | 100.69 | 0.34 |
Derivative spectrophotometry | ||||
30.0 | 98.21 | 1.04 | 98.96 | 1.43 |
40.0 | 97.95 | 1.15 | 98.23 | 1.17 |
50.0 | 100.5 | 0.87 | 97.25 | 1.64 |