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Table 3 Summary of method validation parameters

From: Characterization and toxicity evaluation of degradation products of febantel

Validation parameter

Obtained result

Linearity

Equation of regression line

ay = 39937x + 94778

r2

0.9996

Range (μg mL−1)

50–500

Precision

Actual conc. (μg mL−1)

Intra-day measured conc. (μg mL−1), Mean ± RSD (%)

200.00

300.00

400.00

201.73 ± 0.47

300.93 ± 0.33

400.53 ± 0.18

Actual conc. (μg mL−1)

Inter-day measured conc. (μg mL−1), Mean ± RSD (%)

200.00

300.00

400.00

202.3 ± 01.2

300.86 ± 0.73

399.86 ± 0.49

Accuracy

Spiked conc. (μg mL−1)

Recovery (%), Mean ± RSD (%)

200.00

300.00

400.00

99.80 ± 0.47

99.93 ± 0.80

99.62 ± 0.58

  1. aWhere y = peak area, x = concentration of drug in μg mL−1