Table 2 Parameters used for HPTLC
From: HPTLC analysis of Fumaria parviflora (Lam.) methanolic extract of whole plant
Parameters | Values |
|---|---|
Calibration parameters | |
Calibration mode | Single level |
Statistics mode | CV |
Evaluation mode | Peak height |
Linomat 5 application parameters | |
Spray gas | Inert gas |
Sample solvent type | Methanol |
Dosage speed | 150 nl/s |
Predosage volume | 0.2 μl |
Syringe size | 100 μl |
Application position | 8.0 mm |
Band length | 8.0 mm |
Solvent front position | 75.0 mm |
Detection—CAMAG TLC scanner | |
Number of tracks | 2 |
Position of track X | 15.0 mm |
Distance between tracks | 20.0 mm |
Scan start position Y | 5.0 mm |
Scan end position Y | 75. 0 mm |
Slit dimensions | 6.00 × 0.30 mm, micro |
Optimize optical system | Light |
Scanning speed | 20 mm/s |
Data resolution | 100 μm/step |
Integration: properties | |
Data filtering | Savitsky-Golay 7 |
Baseline correction | Lowest slope |
Peak threshold min. slope | 5 |
Peak threshold min. height | 10 AU |
Peak threshold min. area | 50 |
Peak threshold max. height | 990 AU |
Track start position | 5.0 mm |
Track end position | 75.0 mm |
Display scaling | Automatic |
Measurement | |
Wavelength | 254 nm and 366 nm |
Lamp | D2/Hg |
Measurement type | Remission |
Measurement mode | Absorption/fluorescence |
Optical filter | Second order/K400 |
Detector mode | Automatic |
PM high voltage | 181 V |