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Table 3 Matrix effect and matrix factor for bexarotene at LQC level

From: Development and validation of bexarote by bioanalytical methods using liquid chromatography-tandem mass spectroscopy (LC-MS/MS)

Plasma lot ID Aqueous sample Spiked sample Matrix factor of analyte Matrix factor of IS IS normalized matrix factor Area ratio
Analyte area IS area Analyte area IS area Aqueous sample Spiked sample
MAT6223-I 48,348 1,336,922 45,987 1,317,311 1.08 1.03 1.05 0.0362 0.0349
MAT6224-I 42,001 1,271,968 42,437 1,277,296 0.99 1.00 0.99 0.033 0.0332
MAT6225-I 43,318 1,289,768 40,402 1,257,917 0.95 0.98 0.97 0.0336 0.0321
MAT6220-I 37,838 1,240,867 42,187 1,239,881 0.99 0.97 1.02 0.0305 0.0340
MAT6204-I 41,861 1,246,249 43,795 1,222,111 1.03 0.96 1.07 0.0336 0.0358
MAT6205-I 42,559 1,278,563 41,373 1,221,142 0.97 0.96 1.01 0.0333 0.0339
MATC-0544-XII(H)    43,332 1,289,828 1.02 1.01 1.01   0.0336
MAT6188-IX (L)    42,395 1,282,621 0.99 1.00 0.99   0.0331
MAT6198-(X)-Heparin Plasma    43,054 1,277,420 1.01 1.00 1.01   0.0337
Mean 42,654.16 1,277,389.5   1.003 0.990 1.01333 0.033 0.033
SD   0.037 0.023 0.03082  
%CV 3.76 2.42 3.04