Table 3 Matrix effect and matrix factor for bexarotene at LQC level
Plasma lot ID | Aqueous sample | Spiked sample | Matrix factor of analyte | Matrix factor of IS | IS normalized matrix factor | Area ratio | |||
|---|---|---|---|---|---|---|---|---|---|
Analyte area | IS area | Analyte area | IS area | Aqueous sample | Spiked sample | ||||
MAT6223-I | 48,348 | 1,336,922 | 45,987 | 1,317,311 | 1.08 | 1.03 | 1.05 | 0.0362 | 0.0349 |
MAT6224-I | 42,001 | 1,271,968 | 42,437 | 1,277,296 | 0.99 | 1.00 | 0.99 | 0.033 | 0.0332 |
MAT6225-I | 43,318 | 1,289,768 | 40,402 | 1,257,917 | 0.95 | 0.98 | 0.97 | 0.0336 | 0.0321 |
MAT6220-I | 37,838 | 1,240,867 | 42,187 | 1,239,881 | 0.99 | 0.97 | 1.02 | 0.0305 | 0.0340 |
MAT6204-I | 41,861 | 1,246,249 | 43,795 | 1,222,111 | 1.03 | 0.96 | 1.07 | 0.0336 | 0.0358 |
MAT6205-I | 42,559 | 1,278,563 | 41,373 | 1,221,142 | 0.97 | 0.96 | 1.01 | 0.0333 | 0.0339 |
MATC-0544-XII(H) | 43,332 | 1,289,828 | 1.02 | 1.01 | 1.01 | 0.0336 | |||
MAT6188-IX (L) | 42,395 | 1,282,621 | 0.99 | 1.00 | 0.99 | 0.0331 | |||
MAT6198-(X)-Heparin Plasma | 43,054 | 1,277,420 | 1.01 | 1.00 | 1.01 | 0.0337 | |||
Mean | 42,654.16 | 1,277,389.5 | 1.003 | 0.990 | 1.01333 | 0.033 | 0.033 | ||
SD | 0.037 | 0.023 | 0.03082 | ||||||
%CV | 3.76 | 2.42 | 3.04 | ||||||