Development and validation of bexarote by bioanalytical methods using liquid chromatography-tandem mass spectroscopy (LC-MS/MS)

Future Journal of Pharmaceutical Sciences

Table 5 Back-calculated concentrations of bexarotene for calibration curve standards

Standard ID	A	B	C	D	E	F	G	H	Slope	Intercept	r²
Actual concentration (ng/mL)	1.044	2.7760	10.096	25.240	59.390	131.97	263.950	351.93
PA–01	1.019	2.9590	9.9554	25.592	56.512	134.04	255.361	359.85	0.013	− 0.0002	0.998
PA–02	1.052	2.7224	9.9902	25.479	57.677	132.92	272.270	352.68	0.013	0.0011	0.999
Mean	1.0362	2.8407	9.9728	25.535	57.095	133.48	263.81	356.27
SD	0.02319	0.167301	0.024607	0.079974	0.823921	0.787929	11.956327	5.072289
%CV	2.24	5.89	0.25	0.31	1.44	0.59	4.53	1.42
%Nominal	99.25	102.33	98.78	101.17	96.14	101.14	99.95	101.23