Parameter | Altered | S/N | Resolution | Tailing factor | % RSD |
---|---|---|---|---|---|
Condition | |||||
As such condition (method precision) | 69 | 3.9 | 1.33 | 0.8 | |
Flow variation | Low flow | 51 | 3.5 | 1.39 | 0.6 |
(0.6 mL/min) | (0.5 mL/min) | ||||
High flow | 52 | 3.8 | 1.32 | 0.8 | |
(0.7 mL/min) | |||||
Temperature variation (25 °C) | Low Temperature 20 °C | 60 | 3.5 | 1.35 | 0.6 |
High Temperature 30 °C | 73 | 4.7 | 1.33 | 0.6 | |
Mobile phase Composition variation (IPA) | Low variation | 53 | 3.8 | 1.35 | 0.3 |
n-Heptane: isopropyl alcohol: ethanol: trifluoroacetic acid-(820:150:30:1) v/v/v/v | n-Heptane: isopropyl alcohol: ethanol: trifluoroacetic acid-(835:135:30:1) v/v/v/v | ||||
High variation | 79 | 4.2 | 1.36 | 0.4 | |
n-Heptane: isopropyl alcohol: ethanol: trifluoroacetic acid-(805:165:30:1) v/v/v/v | |||||
Mobile phase Composition variation (ethanol) | Low variation | 73 | 4.1 | 1.39 | 0.8 |
n-Heptane: isopropyl alcohol: ethanol: trifluoroacetic acid-(823:150:27:1) v/v/v/v | |||||
High variation | 65 | 4.3 | 1.41 | 0.6 | |
n-Heptane: isopropyl alcohol: ethanol: trifluoroacetic acid-(817:150:33:1) v/v/v/v |