Table 1 Chromatographic conditions
Equipment | HPLC 3000 series (Analytical Technology ) |
|---|---|
Mobile phase | Methanol to water (9:1) |
Column | C18 (Cosmosil) 250 mm × 4.6ID, particle size 5 μ dimension |
Column temperature | Ambient |
Injection volume | 20 μL |
Flow rate | 0.8 mL/min |
Wavelength | 248 nm |
Sample cooler | 5 °C |