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Table 1 PCR cycle conditions

From: Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines

S. no Steps Apoptosis-related genes and β–actin gene
Temperature Duration Cycles
1. Initial denaturation 94 °C 3 min 1 cycle
2. Denaturation 94 °C 45 s 35 cycles
Primer annealing 52 °C 45 s
Synthesis 72 °C 1 min
3. Final extension 72 °C 7 min 1 cycle
4. Hold at 4 °C