Skip to main content
Future Journal of Pharmaceutical Sciences

Table 1 PCR cycle conditions

From: Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines

S. no

Steps

Apoptosis-related genes and β–actin gene

Temperature

Duration

Cycles

1.

Initial denaturation

94 °C

3 min

1 cycle

2.

Denaturation

94 °C

45 s

35 cycles

Primer annealing

52 °C

45 s

Synthesis

72 °C

1 min

3.

Final extension

72 °C

7 min

1 cycle

4.

Hold at 4 °C

Back to article page