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Table 2 Primer sequences of apoptosis-related genes and β–actin as the control gene

From: Evaluation of chemo-preventive efficacy of Ficus religiosa latex extract by flow cytometry analysis and gene expression studies performed by RT-PCR in various cell lines

S. no Gene name Gene size (bp) Primer sequence (forward and reverse)
1 β–actin 135 5′-CACCATTGGCAATGAGCGGTTC-3′
5′-AGGTCTTTGCGGATGTCCACGT-3′
2 p53 128 5′-CCTGAGCATCTTATCCGAGTGG-3′
5′-TGGATGGTGGTACAGTCAGAGC-3′
3 Caspase3 146 5′-GGAAGCGAATCAATGGACTCTGG-3′
5′-GCATCGACATCTGTACCAGACC-3′
4 Bcl2 127 5′-ATCGCCCTGTGGATGACTGAGT-3′
5′-GCCAGGAGAAATCAAACAGAGGC-3′
5 AKT 155 5′-TGGACTACCTGCACTCGGAGAA-3′
5′-GTGCCGCAAAAGGTCTTCATGG-3′
6 NrF2 112 5′-CACATCCAGTCAGAAACCAGTGG-3′
5′-GGAATGTCTGCGCCAAAAGCTG-3′
7 RelA 135 5′-TGAACCGAAACTCTGGCAGCTG-3′
5′-CATCAGCTTGCGAAAAGGAGCC-3′