Table 19 Robustness study results of methanol

Flow rate (± 0.2 mL/min)

2.3

2.9

5.41

59741

1.3

2.5

2.5

4.93

58451

1.0

2.7

2.2

4.44

57485

1.2

Column oven temperature (± 5 °C)

35 °C

3.0

5.32

59874

1.1

40 °C

2.5

4.93

58451

1.0

45 °C

2.1

4.49

58749

1.2

Vial oven temperature (± 5 °C)

85 °C

2.8

4.91

54758

1.3

90 °C

2.5

4.93

58451

1.0

95 °C

2.6

4.92

58747

1.2