Table 22 Robustness study results of isopropyl alcohol

Flow rate (± 0.2 mL/min)

2.3

2.2

9.07

31248

1.2

2.5

2.0

8.25

32548

1.0

2.7

2.3

7.43

32478

1.3

Column oven temperature (± 5 °C)

35 °C

2.1

9.08

30214

1.2

40 °C

2.0

8.25

32548

1.0

45 °C

2.3

7.45

31067

1.4

Vial oven temperature (± 5 °C)

85 °C

2.1

8.24

30148

1.3

90 °C

2.0

8.25

32548

1.0

95 °C

1.9

8.21

31032

1.1