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Table 1 Mobile phase optimization using different solvents and their mixtures

From: Development and validation by statistical treatment of stability indicating RP-HPLC method for quantification of Orlistat in Orlistat-loaded solid dispersion

Chromatographic parameters Acceptance criteria Resultsa
Mobile phase   A B C D E F
Methanol: buffer 65:44 70:29 75:24 80:19 85:14 90:9
Methanol: chloroform 65:44 70:29 75:24 80:19 85:14 90:9
Methanol : acetonitrile 65:44 70:29 75:24 80:19 85:14 90:9
2% phosphoric acid (%w/v) 1.0 1.0 1.0 1.0 1.0 1.0
Average retention time 7.8 7.2 6.7 6.2 5.9 5.5
Average tailing factor 0.8–1.5 1.6 1.36 1.24 1.13 1.02 1.23
Average number of theoretical plates More than 2000 13,624 12,598 11,234 14,321 13,925 13,268
  1. aData represents mean ± S.D., p < 0.001 by two-way ANOVA