Condition | Recovery % (±SD) | ||
---|---|---|---|
2.5 μg/ml | 5 μg/ml | 7.5 μg/ml | |
HPLC 2 | 97 ± 2.45 | 98 ± 2.76 | 101 ± 1.67 |
Mobile phase with composition 84:15:1 (methanol, acetonitrile and 2% phosphoric acid) | 99 ± 3.51 | 101 ± 2.12 | 102 ± 2.73 |
Flow rate 1.2 ml/min | 98 ± 4.78 | 99 ± 3.85 | 104 ± 3.76 |