Table 1 Chromatographic conditions of developed method
Parameters | Value |
|---|---|
Injection volume | 20 μL |
Column temperature | 27o C |
Detection wavelength | 254 nm |
Flow rate | 1.5 mL/min |
Mobile phase | Phosphate buffer (pH 3):acetonitrile |
Mode of separation | Gradient |
Run time | 45 min |