Skip to main content

Table 1 Chromatographic conditions of developed method

From: Analytical method development and validation for simultaneous estimation of monoammonium glycyrrhizinate and sennoside-B in polyherbal laxative tablet using RP-HPLC

Parameters Value
Injection volume 20 μL
Column temperature 27o C
Detection wavelength 254 nm
Flow rate 1.5 mL/min
Mobile phase Phosphate buffer (pH 3):acetonitrile
Mode of separation Gradient
Run time 45 min