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Table 2 Gradient program of optimized mobile phase

From: Analytical method development and validation for simultaneous estimation of monoammonium glycyrrhizinate and sennoside-B in polyherbal laxative tablet using RP-HPLC

Time % of mobile phase
Solvent A, phosphate buffer (pH 3) Solvent B, acetonitrile
0 95 5
18 55 45
25 20 80
28 20 80
35 55 45
40 95 5
45 95 5