Table 2 Gradient program of optimized mobile phase
Time | % of mobile phase | |
|---|---|---|
Solvent A, phosphate buffer (pH 3) | Solvent B, acetonitrile | |
0 | 95 | 5 |
18 | 55 | 45 |
25 | 20 | 80 |
28 | 20 | 80 |
35 | 55 | 45 |
40 | 95 | 5 |
45 | 95 | 5 |
Time | % of mobile phase | |
|---|---|---|
Solvent A, phosphate buffer (pH 3) | Solvent B, acetonitrile | |
0 | 95 | 5 |
18 | 55 | 45 |
25 | 20 | 80 |
28 | 20 | 80 |
35 | 55 | 45 |
40 | 95 | 5 |
45 | 95 | 5 |