Table 5 Robustness study

Mobile phase ratio

70:30

0.74

97.89

1.64

8254

1.05

98.38

1.65

5957

65:35

0.87

98.06

1.74

8102

1.16

99.35

1.65

6264

60:40

1.02

96.54

1.72

7650

1.82

97.64

1.61

6441

Mean ± SD

97.49±0.83

98.45±0.85

RSD (%)

0.85

0.87

Flow rate (mL/min)

0.8

1.14

97.49

1.65

6845

1.71

98.14

1.62

6038

1.0

0.87

98.26

1.65

8125

1.16

99.52

1.71

6506

1.2

0.72

96.07

1.61

7845

1.03

97.84

1.65

6382

Mean ± SD

97.27±1.11

98.5±0.89

RSD (%)

1.14

0.9

Column temperature (°C)

35 °C

0.85

99.04

1.70

8201

1.12

98.47

1.64

6218

30 °C

0.87

98.63

1.71

8054

1.16

99.04

1.64

6151

25 °C

0.89

97.82

1.71

7654

1.19

97.22

1.62

6424

Mean ± SD

98.49±0.62

98.24±0.93

RSD (%)

0.63

0.94

UV wavelength

258

0.87

96.14

1.71

7951

1.14

99.81

1.63

5984

254

0.87

98.36

1.72

8156

1.16

98.74

1.61

6068

250

0.85

97.03

1.71

7899

1.17

96.28

1.61

6248

Mean ± SD

97.17±1.11

98.27±1.81

RSD (%)

1.14

1.84