Future Journal of Pharmaceutical Sciences

Table 3 Results of method validation

From: Multivariate optimization of liquid chromatographic conditions for determination of dapagliflozin and saxagliptin, application to an in vitro dissolution and stability studies

Parameter	Dapagliflozin			Saxagliptin
Precision
Repeatability	Peak area	SD	% RSD	Peak area	SD	% RSD
Intra-day	1989124	21017	1.05	3584561	21476	0.599
Inter-day	2018092	23326	1.15	3551248	26363	0.742
Accuracy
Recovery level	Amount Spiked (μg/mL)	% Recovery	% RSD	Amount Spiked (μg/mL)	% Recovery	% RSD
80%	80	100.3	0.16	40	100.6	0.39
100%	100	100.2	0.10	50	100.9	0.40
120%	120	100.1	0.10	60	100.3	0.57
System suitability
Retention time	3.5			5.0
Peak area	515956			602550
Tailing factor	1.3			1.2
Theoretical plates	6822			9773
Capacity factor (K’)	2.5			4.0
Selectivity factor (α)	1.6
Resolution	7.3

SD standard deviation, RSD relative standard deviation (n=6)

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