Multivariate optimization of liquid chromatographic conditions for determination of dapagliflozin and saxagliptin, application to an in vitro dissolution and stability studies

Future Journal of Pharmaceutical Sciences

Table 4 Robustness data of optimized method

Factor	Change condition	Dapagliflozin (%RSD)		Saxagliptin (%RSD)
Factor	Change condition	Rt	Tp	Rt	Tp
Flow rate (mL/min)	0.92	1.02	0.78	1.12	0.57
	0.96	0.31	0.72	0.47	0.41
	1.0	0.56	1.04	0.88	0.86
Organic phase (%)	25	1.11	0.85	1.11	0.66
	26	0.52	0.71	0.24	0.52
	27	0.57	0.83	0.84	0.69
Buffer pH	5.7	0.84	0.68	0.13	0.64
	5.8	0.21	0.71	0.46	0.58
	5.9	0.53	0.76	0.82	0.87