Table 10 Comparison between the present method with the reported method
Parameter | Present method | Reported method | ||
|---|---|---|---|---|
Sitagliptin | Ertugliflozin | Sitagliptin | Ertugliflozin | |
Retention time | 0.859min | 1.570min | 4.60 min | 2.39min |
LOD | 0.5μg/ml | 0.13μg/ml | 12.71μg/ml | 42.37μg/ml |
LOQ | 1.53μg/ml | 0.38μg/ml | 8.59μg/ml | 28.65μg/ml |
Mobile composition | Acetonitrile to water (pH 3.5) (50:50%, v/v) | 0.5 mM potassium dihydrogen ortho phosphate buffer: Methanol (55:45 v/v) | ||
Flow rate | 0.2ml/min | 1 ml/min | ||
Column | C8(100×2.1mm, 2μm) | C18 (250×4.6 mm, 5 μm) | ||