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Table 1 Optimized chromatographic conditions

From: Development and validation of stability-indicating RP-HPLC method for estimation of dalfampridine in bulk drug and tablet dosage form

Column Waters XTerra® RP 18 5 μm 4.6 × 250 mm
Flow rate 0.5 mL/min
Injection volume 5 μL
Wavelength 262 nm
Column temp 40 °C
Autosampler temp 25 °C
Run time 10.0 min
Retention time 4.4 min
Mobile phase Buffer pH 3.0: acetonitrile (60:40)
Theoretical plates 6510
Tailing factor 1.1