Table 1 Optimized chromatographic conditions
Column | Waters XTerra® RP 18 5 μm 4.6 × 250 mm |
|---|---|
Flow rate | 0.5 mL/min |
Injection volume | 5 μL |
Wavelength | 262 nm |
Column temp | 40 °C |
Autosampler temp | 25 °C |
Run time | 10.0 min |
Retention time | 4.4 min |
Mobile phase | Buffer pH 3.0: acetonitrile (60:40) |
Theoretical plates | 6510 |
Tailing factor | 1.1 |