|
Parameters
|
Values
|
Retention time
|
Tailing factor
|
Theoretical plates
|
RSD of standard area (%)
|
% Assay
|
Absolute difference
|
|---|
|
Control
|
As per method
|
4.990
|
1.20
|
6760
|
0.16
|
100.5
|
–
|
|
Flow rate (± 0.1 mL/min)
|
0.4 mL/min
|
6.201
|
1.18
|
7371
|
0.22
|
100.5
|
0.0
|
|
0.6 mL/min
|
4.153
|
1.20
|
5922
|
0.26
|
100.2
|
0.3
|
|
Change in wavelength (± 5 nm)
|
257 nm
|
4.967
|
1.21
|
6608
|
0.34
|
100.5
|
0.0
|
|
267 nm
|
4.967
|
1.21
|
6656
|
0.14
|
98.9
|
1.6
|
|
Buffer pH (± 0.2 unit)
|
pH − 2.98
|
4.912
|
1.20
|
6230
|
0.25
|
98.7
|
1.8
|
|
pH − 3.02
|
4.922
|
1.0
|
5998
|
0.68
|
98.6
|
1.9
|
|
Column temperature (± 5 °C)
|
35 °C
|
4.962
|
1.20
|
6522
|
0.57
|
99.6
|
0.9
|
|
45 °C
|
3.781
|
1.20
|
6759
|
0.39
|
99.7
|
0.8
|
