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Table 4 Result of robustness 

From: Development and validation of stability-indicating RP-HPLC method for estimation of dalfampridine in bulk drug and tablet dosage form

Parameters Values Retention time Tailing factor Theoretical plates RSD of standard area (%) % Assay Absolute difference
Control As per method 4.990 1.20 6760 0.16 100.5
Flow rate (± 0.1 mL/min) 0.4 mL/min 6.201 1.18 7371 0.22 100.5 0.0
0.6 mL/min 4.153 1.20 5922 0.26 100.2 0.3
Change in wavelength (± 5 nm) 257 nm 4.967 1.21 6608 0.34 100.5 0.0
267 nm 4.967 1.21 6656 0.14 98.9 1.6
Buffer pH (± 0.2 unit) pH − 2.98 4.912 1.20 6230 0.25 98.7 1.8
pH − 3.02 4.922 1.0 5998 0.68 98.6 1.9
Column temperature (± 5 °C) 35 °C 4.962 1.20 6522 0.57 99.6 0.9
45 °C 3.781 1.20 6759 0.39 99.7 0.8