Table 4 Robustness evaluation
Independent variables | Explored range | LVD | TDF | Optimized value | ||||
|---|---|---|---|---|---|---|---|---|
Tailing factor | Retention time (Rt) | Theoretical plates | Tailing factor | Retention time (Rt) | Theoretical plates | |||
Flow rate (mL/min) | 0.8–1.2 | 1.36 | 4.59 | 7895 | 1.17 | 8.45 | 2549 | 1 mL/min |
1.30 | 4.60 | 7896 | 1.14 | 8.50 | 2534 | |||
1.45 | 4.61 | 7894 | 1.15 | 8.53 | 2541 | |||
Column oven temperature (oC) | 25–35 | 1.35 | 4.56 | 7859 | 1.14 | 8.35 | 2514 | 30 oC |
1.34 | 4.62 | 7887 | 1.12 | 8.38 | 2516 | |||
1.33 | 4.59 | 7864 | 1.15 | 8.34 | 2534 | |||
pH of solvent system | 3.0–4.0 | 1.33 | 4.57 | 7814 | 1.16 | 8.39 | 2536 | pH 4.0 |
1.29 | 4.55 | 7858 | 1.15 | 8.41 | 2574 | |||
1.36 | 4.61 | 7865 | 1.17 | 8.38 | 2539 | |||