Table 1 Phytochemical analysis summary of root extract of Strobilanthes heyneanus

Alkaloids

Hager’s test: To the extract, a saturated aqueous solution of picric acid was added and shaken well.

Formation of yellow precipitate

+

Steroids

Liebermann-Burchard’s test: The extract was mixed with 1 mL of acetic anhydride and shaken well. To this, few drops of conc. H₂SO₄ were added along the sides of the test tube.

Appearance of dark violet colour

+

Terpenoids

Salkowski test: To the extract, chloroform was added and mixed well. Then, a few drops of conc. H₂SO₄ were added along the sides of the test tube.

Appearance of red ring

+

Phenols

FeCl₃ test: To the extract, a few drops of 5% FeCl₃ solution was added and shaken well.

Appearance of violet colour

+

Flavonoids

Alkaline reagent test: To the extract, a few drops of 2% NaOH solution was added and shaken well.

Appearance of yellow colour

+

Tannins

Lead acetate test: To the extract, a few drops of 5% Pb (CH₃COO)₂ solution was added and shaken well.

Appearance of white colour

+

Saponins

Foam test: To the extract, 3 mL of distilled water was added and shaken vigorously.

Appearance of foam

+

Glycosides

Legal’s test: To the extract, few drops of pyridine and few drops of alkaline sodium nitroprusside solution was added and shaken well.

Appearance of blood red colour

+

Carbohydrates

Molisch test: To the extract, two drops of alcoholic α-naphthol solution was added and shaken well. To this, a few drops of conc. H₂SO₄ was added.

Appearance of violet ring

+