Treatment | Concentration (μg/ml) | Meann percentage inhibition of radical ± S.D. | IC50 values (μg/ml) |
---|---|---|---|
DPPH Method | |||
Rutin | 2 | 28.56 ± 0.321 | 5.45 |
4 | 40.25 ± 0.450 | ||
6 | 55.66 ± 0.789 | ||
8 | 65.49 ± 0.630 | ||
10 | 77.80 ± 0.499 | ||
12 | 87.69 ± 0.652 | ||
F1 | 5 | 16.55 ± 0.458 | 73.26 |
10 | 18.24 ± 0.658 | ||
20 | 25.65 ± 0.258 | ||
40 | 36.22 ± 0.459 | ||
80 | 55.01 ± 0.658 | ||
160 | 89.57 ± 0.448 | ||
Hydrogen peroxide method | |||
Ascorbic acid | 0.0625 | 16.25 ± 0.658 | 0.89 |
0.125 | 20.50 ± 0.785 | ||
0.25 | 26.44 ± 0.985 | ||
0.50 | 35.69 ± 0.756 | ||
1 | 56.52 ± 0.325 | ||
2 | 91.25 ± 0.752 | ||
F1 | 0.0625 | 11.24 ± 0.550 | 1.30 |
0.125 | 13.49 ± 0.456 | ||
0.25 | 17.98 ± 0.325 | ||
0.50 | 25.64 ± 0.489 | ||
1 | 42.66 ± 0.653 | ||
2 | 70.55 ± 0.324 | ||
Nitric oxide method | |||
Ascorbic acid | 10 | 45.14 ± 0.658 | 27.03 |
20 | 49.58 ± 0.789 | ||
40 | 52.32 ± 0.654 | ||
80 | 59.44 ± 0.125 | ||
160 | 72.44 ± 0.315 | ||
320 | 96.47 ± 0.458 | ||
F1 | 10 | 30.14 ± 0.985 | 121.85 |
20 | 32.25 ± 0.456 | ||
40 | 36.44 ± 0.325 | ||
80 | 43.55 ± 0.458 | ||
160 | 58.12 ± 0.659 | ||
320 | 82.44 ± 0.311 |