Table 5 Optimized chromatographic conditions
Parameter/conditions | Description/values |
|---|---|
Column | XTERRA RP18 (250 × 4.6 mm, 5 μ) |
Detector | UV-Vis detector |
Flow rate | 1.0 mL/min |
Injection volume | 20 μl |
Wavelength | 260 nm |
Column temperature | 25 °C |
Run time | 15 min |
Buffer | Phosphate buffer ( pH adjusted to 5.0) |
Mobile phase | ACN: buffer (50:50) |
Program | Isocratic |