Factors | Chromatographic changes | ||
---|---|---|---|
Flow rate (ml/min) | Level | Retention time | Tailing factor |
0.9 | −0.1 | 4.77 | 1.14 |
1.0 | 0 | 4.79 | 1.15 |
1.1 | + 0.1 | 4.78 | 1.16 |
Mean | 4.78 | 1.15 | |
S. D. | ± 0.010 | ± 0.010 | |
Mobile phase (v/v/v) | |||
44:56 | −1.0 | 4.77 | 1.16 |
45:55 | 0 | 4.79 | 1.15 |
46:54 | + 1.0 | 4.83 | 1.17 |
Mean | 4.79 | 1.16 | |
S. D. | ± 0.030 | ± 0.010 | |
Wavelength (nm) | |||
261 | −1.0 | 4.79 | 1.14 |
262 | 0 | 4.79 | 1.15 |
263 | + 1.0 | 4.78 | 1.15 |
Mean | 4.78 | 1.14 | |
S. D. | ± 0.005 | ± 0.005 |