Table 1 Optimized chromatographic conditions
Parameters | Results |
|---|---|
Elution | Isocratic |
Mobile phase | 0.1 % trifluroacetic acid in 1000 ml water:acetonitrile (50:50) |
Column | Agilent Eclipse XDB C18, 4.6 × 250 mm, 5μ |
Flow rate | 1.0 ml/min |
Detection | 260.0 nm |
Injection volume | 20 μl |
Temperature | Ambient |
Retention time | 3.674 min for sofosbuvir and 5.704 min for phosphoryl impurity |
Run time | 25 min |