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Table 10 Robustness studies (different column) for standard and test solutions of sofosbuvir

From: Development and validation of RP HPLC method for the estimation of Sofosbuvir and related impurity in bulk and pharmaceutical dosage form

Injection no.

Peak area (AU)

Retention time (min)

Standard

Test

Standard

Test

1

5992287

6004074

3.766

3.770

2

6001913

5996533

3.766

3.769

3

5998929

6007984

3.768

3.768

Mean

5997710

6002864

3.767

3.769

Std. dev

4927.48

5820.66

0.001

0.001

% RSD

0.082

0.097

0.028

0.027

% Assay

99.60