Table 2 Experimental results and selected method conditions
Parameter/condition | Description |
|---|---|
Injection volume | 20 μL |
Wavelength | 216 nm |
Mobile phase | Acetonitrile: buffer (65:35% v/v) |
Program | Isocratic |
Flow rate | 1.0 mL/min |
Column oven temp | 30° C |
Run time | 7 min |
Buffer | 10 mM potassium dihydrogen orthophosphate in water. Adjusted to a pH 2.5 by orthophosphoric acid |