Table 1 Optimized chromatographic conditions for the analysis of vildagliptin by RP-HPLC
From: Bioequivalence study of different brands of vildagliptin in healthy human subjects
Drug | Time (min) | Mobile buffer (%A) | Phase ACN (%B) | Flow rate (ml/min) | Detection wavelength (nm) | Injection volume (μl) | Retention time (min) |
|---|---|---|---|---|---|---|---|
Vildagliptin | 0 | 95 | 5 | ||||
5 | 80 | 20 | |||||
10 | 50 | 50 | |||||
15 | 20 | 80 | 1 | 210 | 20 | 11 | |
17 | 5 | 95 | |||||
19 | 95 | 5 | |||||
20 | 95 | 5 |