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Table 5 Parameters for analytical method development for in vivo estimation of trospium chloride

From: Development, optimization and pharmacokinetic evaluation of biphasic extended-release osmotic drug delivery system of trospium chloride for promising application in treatment of overactive bladder

  Chromatographic specifications Mass spectrometric specifications
1 Stationary Phase Zorbax SB C18, 75 × 2.1 mm, 3.5 µm with guard column MRM transition (amu) 392.2 > 182
2 Mobile Phase Mixture of Buffer: Organic mixture (20:80; v/v) Declustering potential (V) 120
3 Organic mixture Acetonitrile: Methanol (95:5; v/v) Entrance potential (V) 8
4 Flow rate 0.3 mL/min Collision energy (V) 41
5 Auto-injector temperature 5 ± 1 °C Collision cell exit potential (V) 8
6 Column temperature 30 ± 1 °C Dwell time (ms) 300
7 Injection volume 5 µL    -
8 Run time 3.5 min  -  -
9 Detector Triple quadrupole mass spectrometer  -  -