Table 5 Parameters for analytical method development for in vivo estimation of trospium chloride
Chromatographic specifications | Mass spectrometric specifications | |||
|---|---|---|---|---|
1 | Stationary Phase | Zorbax SB C18, 75 × 2.1 mm, 3.5 µm with guard column | MRM transition (amu) | 392.2 > 182 |
2 | Mobile Phase | Mixture of Buffer: Organic mixture (20:80; v/v) | Declustering potential (V) | 120 |
3 | Organic mixture | Acetonitrile: Methanol (95:5; v/v) | Entrance potential (V) | 8 |
4 | Flow rate | 0.3 mL/min | Collision energy (V) | 41 |
5 | Auto-injector temperature | 5 ± 1 °C | Collision cell exit potential (V) | 8 |
6 | Column temperature | 30 ± 1 °C | Dwell time (ms) | 300 |
7 | Injection volume | 5 µL | - | |
8 | Run time | 3.5 min | - | - |
9 | Detector | Triple quadrupole mass spectrometer | - | - |