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Table 2 Rf values of various biomarkers and Anu Taila

From: Identification and estimation of bioactive constituents Negundoside, Berberine chloride, and Marmelosin by HPLC and HPTLC for development of quality control protocols for Ayurvedic medicated oil formulation

S. no.

Tracks

At UV 254 nm

At UV 366 nm

After derivatization

1

Rf

Colour

Rf

Colour

Rf

Colour

2

Track 2: PHBA

0.08

Green

0.08

Blue

–

–

3

Track 3: Embelin

0.13

Green

0.13

Black

0.13

Yellow

4

Track 4: Marmelosin

0.41

Green

0.41

Blue

0.41

Yellowish gray

5

Track 1 and 5:

Anu Taila

0.05

Green

0.05, 0.10

0.13, 0.24

0.35

Blue

0.04, 0.13

Violet

6

 

0.10, 0.13

Blue

  

0.18, 0.24

0.29, 0.32

0.38

Gray

7

 

0.18, 0.25,

0.35, 0.41,

0.45, 0.54,

0.61

Green

  

0.45, 0.48

0.54, 0.88

Violet

8

Track 6: Berberine chloride

–

–

–

–

0.24

Gray

9

Track 7: Solasodine

0.52

Green

–

–

0.52

Gray

10

Track 8: Lupeol

–

–

–

–

0.45

Violet

11

Track 9: Glycyrrhizin

–

–

0.57

Blue

–

–