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Table 1 Suggested experiments by design of experiments and responses

From: Design of experiment-driven stability-indicating RP-HPLC method for the determination of tofacitinib in nanoparticles and skin matrix

Variable 1

Variable 2

Response 1

Response 2

Organic phase

Buffer pH

Retention time

Tailing factor

%

–

Min

–

40

3.5

7.243

1.681

50

3.5

4.918

1.514

60

3.5

3.667

1.325

40

4.5

9.790

1.652

50

4.5

5.430

1.473

60

4.5

3.868

1.352

40

5.5

11.168

1.435

50

5.5

5.630

1.365

60

5.5

3.902

1.334

50

4.5

5.420

1.472

50

4.5

5.430

1.455